Research Article
Identification and Validation of Reference Genes for RT-qPCR Normalization in Mythimna separata (Lepidoptera: Noctuidae)
Table 1
Primer pairs of candidate reference genes used for RT-qPCR analysis.
| | Gene symbol | Gene name | Accession number | Sequence | Product length (bp) | Primer efficiency (%) | R2 |
| | GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | HM0055756.1 | F: CGCTACAGTCGTTGCCATCA | 126 | 105.8 | 0.998 | | R: ACGACGAGGAAGCCATCTTG | | 18S | 18S ribosomal | MG654665 | F: GGCCGTTCTTAGTTGGTGGA | 99 | 106.1 | 0.998 | | R: AGCCACGCACACCTAAATGA | | 28S | 28S ribosomal | MG654666 | F: GCAGCGGAACCGTTTCAATA | 134 | 98.80 | 1.000 | | R: ATGGAACTCGAACGCTCAGG | | EF | Elongation factor1 alpha | KR869784.1 | F: AAGAAATCTGCCCGCGGTAT | 72 | 102.1 | 0.991 | | R: TGCGGTTTAGCGATGGAAGT | | RPL12 | Ribosomal protein L12 | MG665388 | F: ACGTTTGATTGCAGTTCGCC | 102 | 100.9 | 0.995 | | R: TTAGGCGGCATTGTGCTGTA | | β-TUB | Beta-Tubulin | EU234504.1 | F: CGAGCTTGTGGATTCCGTCT | 91 | 108.9 | 0.992 | | R: GCCGAGAGAGTGTGTGAGTT | | ATPase | ATP synthase subunit H | KC683729.1 | F: AACAACCCTCGATGCTAGGC | 98 | 102.5 | 0.997 | | R: CCGACACCGTCTTGTAGGTC | | TBP | TATA box binding protein | MG657270 | F: GGCACTACAACAAGCACATGG | 86 | 98.3 | 0.994 | | R: CTCGTGGCCGTACCATTCTG | | β-ACT | Beta-Actin | GQ856238.1 | F: GTCCTACGAACTTCCCGACG | 95 | 96.8 | 0.997 | | R: CCATACCCAGGAATGAGGGC | | CypA | Cyclophilin A | HM113489.1 | F: GCTCAGAACAGGTGTGACCC | 87 | 101.1 | 0.998 | | R: GTGGAGCCCTTGTAACCGAA | | PKG | cGMP-dependent protein kinase | GQ844298 | F: TTACCGGCTCACCACCTTTC | 182 | 99.1 | 0.998 | | R: CTCTGTGATGCCACCTCGTT |
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