Identification and Validation of Reference Genes for RT-qPCR Normalization in Mythimna separata (Lepidoptera: Noctuidae)

Table 3

Expression stabilities of the candidate reference genes under different abiotic conditions.

Abiotic condition	Reference gene	geNorm		Normfinder		BestKeeper		△Ct
		Stability	Rank	Stability	Rank	Stability	Rank	Stability	Rank
Photoperiod	GAPDH	0.179	1	1.657	6	0.58	4	1.97	4
	18S	2.504	10	5.034	10	5.08	10	5.07	10
	28S	1.863	9	3.608	9	3.94	9	3.96	9
	EF	0.581	6	0.059	1	0.27	2	1.82	1
	RPL12	0.293	4	1.188	4	0.25	1	1.83	2
	β-TUB	0.963	7	0.117	2	1.31	7	2.09	6
	ATPase	1.169	8	0.117	3	1.43	8	2.14	7
	TBP	0.408	5	0.177	8	0.97	6	2.32	8
	β-ACT	0.179	1	2.177	7	0.61	5	1.98	5
	CypA	0.228	3	1.696	5	0.42	3	1.88	3
Temperature	GAPDH	0.704	5	1.396	5	0.77	5	1.96	4
	18S	2.689	10	5.316	10	4.99	10	5.36	10
	28S	2.021	9	5.064	9	4.79	9	5.17	9
	EF	0.43	3	0.173	3	0.21	1	1.86	2
	RPL12	0.537	4	0.669	4	0.33	3	1.85	1
	β-TUB	0.181	1	0.09	1	0.32	2	1.94	3
	ATPase	0.181	1	0.09	2	0.43	4	2	5
	TBP	0.946	8	2.327	8	1.45	8	2.5	8
	β-ACT	0.853	7	1.931	7	1.15	7	2.23	7
	CypA	0.767	6	1.539	6	0.86	6	2.02	6
Abiotic treatment	GAPDH	0.384	3	1.458	6	0.68	4	1.94	4
	18S	2.546	10	4.937	10	5.04	10	5.01	10
	28S	1.931	9	4.222	9	4.36	9	4.45	9
	EF	0.689	6	0.238	3	0.30	2	1.81	1
	RPL12	0.471	4	0.917	4	0.29	1	1.82	2
	β-TUB	0.938	7	0.160	1	0.81	5	2.00	5
	ATPase	1.084	8	0.160	2	0.93	7	2.08	7
	TBP	0.557	5	2.184	8	1.21	8	2.36	8
	β-ACT	0.350	1	1.760	7	0.88	6	2.07	6
	CypA	0.350	1	1.455	5	0.64	3	1.93	3