Identification and Validation of Reference Genes for RT-qPCR Normalization in Mythimna separata (Lepidoptera: Noctuidae)

Table 4

Expression stabilities of the candidate reference genes of all samples.

Abiotic condition	Reference gene	geNorm		Normfinder		BestKeeper		△Ct
		Stability	Rank	Stability	Rank	Stability	Rank	Stability	Rank
All samples	GAPDH	1.148	5	1.242	6	1.14	5	2.14	5
	18S	2.102	9	3.049	9	3.38	10	3.5	9
	28S	2.49	10	3.759	10	3.29	9	4.04	10
	EF	0.823	3	0.779	2	0.85	1	1.89	2
	RPL12	0.625	1	1.005	4	1.1	3	1.96	4
	β-TUB	0.97	4	0.209	1	1.37	7	1.88	1
	ATPase	1.408	7	0.926	3	1.28	6	2.14	6
	TBP	1.274	6	1.939	7	1.12	4	2.45	7
	β-ACT	1.68	8	2.437	8	2.35	8	2.94	8
	CypA	0.625	1	1.068	2	0.99	2	1.95	3