Identification and Validation of Reference Genes for RT-qPCR Normalization in Mythimna separata (Lepidoptera: Noctuidae)

Table 5

The first three most stable reference genes recommended by RefFinder under various experimental conditions.

Biotic and abiotic condition		Reference gene
Biotic factors	Developmental stages	EF	CypA	β-TUB
	Larval tissues	EF	CypA	RPL12
	Adult tissues	EF	TBP	β-TUB
	Densities	RPL12	β-TUB	EF
	All biotic samples	EF	β-TUB	CypA
Abiotic stress	Photoperiod treatments	EF	RPL12	GADPH
	Temperature treatments	β-TUB	EF	ATPase
	All abiotic samples	RPL12	EF	CypA
All samples		EF	β-TUB	CypA