BioMed Research International / 2018 / Article / Tab 2 / Research Article
Protective Effects of Benzoic Acid, Bacillus Coagulans, and Oregano Oil on Intestinal Injury Caused by Enterotoxigenic Escherichia coli in Weaned Piglets Table 2 Primer sequences used for real-time PCR.
Primer Primer sequence (5′ –3′) Anneal temperature (°C) Product length (bp) GeneBank accession No. GAPDH F:TGAAGGTCGGAGTGAACGGAT R:CACTTTGCCAGAGTTAAAAGCA 55.7 114 NM_001206359.1 Claudin-1 F: GCCACAGCAAGGTATGGTAAC R: AGTAGGGCACCTCCCAGAAG 59.0 140 FJ873109.1 Occludin F: CTACTCGTCCAACGGGAAAG R:ACGCCTCCAAGTTACCACTG 59.0 158 NM_001163647.2 ZO-1 F: CAGCCCCCGTACATGGAGA R: GCGCAGACGGTGTTCATAGTT 59.0 114 XM_005659811 Mucin1 F: GTGCCGCTGCCCACAACCTG R:AGCCGGGTACCCCAGACCCA 59.0 141 XM_001926883.4 Mucin2 F:GGTCATGCTGGAGCTGGACAGT R:TGCCTCCTCGGGGTCGTCAC 59.0 181 XM_003122394.1 TLR4 F: TTACAGAAGCTGGTTGCCGT R:TCCAGGTTGGGCAGGTTAGA 63.3 152 GQ304754 CD14 F: CCTCAGACTCCGTAATGTG R: CCGGGATTGTCAGATAGG 59.0 180 AB267810 MYD88 F: CCATTCGAGATGACCCCCTG R: TAGCAATGGACCAGACGCAG 59.0 183 NM_001099923.1 TRIF F: CAAGTGGAGGAAGGAACAGG R: CAACTGCGTCTGGTAGGACA 59.0 139 XM_003362039.1 IRAK1 F: CAAGGCAGGTCAGGTTTCGT R:TTCGTGGGGCGTGTAGTGT 55.7 115 XM_003135490.1 TRAF6 F: CAAGAGAATACCCAGTCGCACA R: ATCCGAGACAAAGGGGAAGAA 55.7 122 NM-001105286.1 P38 MAPK F:AGTTGAAGCTCATTTTAAGACTCGT R: AGTTCATCTTCGGCATCTGGG 55.7 117 XM_001929490.5 NF-κ Bp65 F: GTGTGTAAAGAAGCGGGACCT R: CACTGTCACCTGGAAGCAGAG 55.7 139 EU399817.1 IL-1β F: CAGCTGCAAATCTCTCACCA R: TCTTCATCGGCTTCTCCACT 55.7 112 NM_214055.1 TNF-α F: CGTGAAGCTGAAAGACAACCAG R: GATGGTGTGAGTGAGGAAAACG 55.7 121 NM_214022.1 NOD1 F: CTGTCGTCAACACCGATCCA R: CCAGTTGGTGACGCAGCTT 55.7 57 AB187219.1 NOD2 F: GAGCGCATCCTCTTAACTTTCG R: ACGCTCGTGATCCGTGAAC 55.7 66 AB195466.1 RIPK2 F: CAGTGTCCAGTAAATCGCAGTTG R: CAGGCTTCCGTCATCTGGTT 59.0 206 XM_003355027.1
GAPDH: glyceraldehyde 3-phosphate dehydrogenase; TLR4: toll-like receptor 4; MYD88: myeloid differentiation factor 88; IRAK1: IL-1 receptor-associated kinase 1; TRAF6: TNF receptor-associated factor 6; P38 MAPK: P38 mitogen-activated protein kinase; NF-κ Bp65: nuclear factor-κ B p65; IL-1β : interleukin-1β ; TNF-α : tumor necrosis factor-α ; NOD: nucleotide-binding oligomerization domain protein; RIPK2: receptor-interacting serine/threonine-protein kinase 2.