BioMed Research International

Research Article

Protective Effects of Benzoic Acid, Bacillus Coagulans, and Oregano Oil on Intestinal Injury Caused by Enterotoxigenic Escherichia coli in Weaned Piglets

Table 2

Primer sequences used for real-time PCR.


Primer	Primer sequence (5′ –3′)	Anneal temperature (°C)	Product length (bp)	GeneBank accession No.

GAPDH	F:TGAAGGTCGGAGTGAACGGAT R:CACTTTGCCAGAGTTAAAAGCA	55.7	114	NM_001206359.1
Claudin-1	F: GCCACAGCAAGGTATGGTAAC R: AGTAGGGCACCTCCCAGAAG	59.0	140	FJ873109.1
Occludin	F: CTACTCGTCCAACGGGAAAG R:ACGCCTCCAAGTTACCACTG	59.0	158	NM_001163647.2
ZO-1	F: CAGCCCCCGTACATGGAGA R: GCGCAGACGGTGTTCATAGTT	59.0	114	XM_005659811
Mucin1	F: GTGCCGCTGCCCACAACCTG R:AGCCGGGTACCCCAGACCCA	59.0	141	XM_001926883.4
Mucin2	F:GGTCATGCTGGAGCTGGACAGT R:TGCCTCCTCGGGGTCGTCAC	59.0	181	XM_003122394.1
TLR4	F: TTACAGAAGCTGGTTGCCGT R:TCCAGGTTGGGCAGGTTAGA	63.3	152	GQ304754
CD14	F: CCTCAGACTCCGTAATGTG R: CCGGGATTGTCAGATAGG	59.0	180	AB267810
MYD88	F: CCATTCGAGATGACCCCCTG R: TAGCAATGGACCAGACGCAG	59.0	183	NM_001099923.1
TRIF	F: CAAGTGGAGGAAGGAACAGG R: CAACTGCGTCTGGTAGGACA	59.0	139	XM_003362039.1
IRAK1	F: CAAGGCAGGTCAGGTTTCGT R:TTCGTGGGGCGTGTAGTGT	55.7	115	XM_003135490.1
TRAF6	F: CAAGAGAATACCCAGTCGCACA R: ATCCGAGACAAAGGGGAAGAA	55.7	122	NM-001105286.1
P₃₈MAPK	F:AGTTGAAGCTCATTTTAAGACTCGT R: AGTTCATCTTCGGCATCTGGG	55.7	117	XM_001929490.5
NF-κBp65	F: GTGTGTAAAGAAGCGGGACCT R: CACTGTCACCTGGAAGCAGAG	55.7	139	EU399817.1
IL-1β	F: CAGCTGCAAATCTCTCACCA R: TCTTCATCGGCTTCTCCACT	55.7	112	NM_214055.1
TNF-α	F: CGTGAAGCTGAAAGACAACCAG R: GATGGTGTGAGTGAGGAAAACG	55.7	121	NM_214022.1
NOD1	F: CTGTCGTCAACACCGATCCA R: CCAGTTGGTGACGCAGCTT	55.7	57	AB187219.1
NOD2	F: GAGCGCATCCTCTTAACTTTCG R: ACGCTCGTGATCCGTGAAC	55.7	66	AB195466.1
RIPK2	F: CAGTGTCCAGTAAATCGCAGTTG R: CAGGCTTCCGTCATCTGGTT	59.0	206	XM_003355027.1

GAPDH: glyceraldehyde 3-phosphate dehydrogenase; TLR4: toll-like receptor 4; MYD88: myeloid differentiation factor 88; IRAK1: IL-1 receptor-associated kinase 1; TRAF6: TNF receptor-associated factor 6; P₃₈MAPK: P₃₈ mitogen-activated protein kinase; NF-κBp65: nuclear factor-κB p65; IL-1β: interleukin-1β; TNF-α: tumor necrosis factor-α; NOD: nucleotide-binding oligomerization domain protein; RIPK2: receptor-interacting serine/threonine-protein kinase 2.