Protective Effects of Benzoic Acid, Bacillus Coagulans, and Oregano Oil on Intestinal Injury Caused by Enterotoxigenic Escherichia coli in Weaned Piglets

Table 3

Primer and probe sequences used for real-time PCR.

Items	Primer and probe sequence (5′ –3′)	Anneal temperature (°C)	Product length (bp)
Total bacteria	F: ACTCCTACGGGAGGCAGCAG; R: ATTACCGCGGCTGCTGG	57.9	200
Lactobacillus	F: GAGGCAGCAGTAGGGAATCTTC; R: CAACAGTTACTCTGACACCCGTTCTTC; P: AAGAAGGGTTTCGGCTCGTAAAACTCTGTT	53.0	126
Bifidobacterium	F: CGCGTCCGGTGTGAAAG; R: CTTCCCGATATCTACACATTCCA; P: ATTCCACCGTTACACCGGAA	57.9	121
Bacillus	F:GCAACGAGCGCAACCCTTGA; R:TCATCCCCACCTTCCTCCGGT; P: CGGTTTGTCACCGGCAGTCACCT	53.0	92
Escherichia coli	F: CATGCCGCGTGTATGAAGAA; R: CGGGTAACGTCAATGAGCAAA; P: AGGTATTAACTTTACTCCCTTCCTC	53.0	96