Protective Effects of Benzoic Acid, Bacillus Coagulans, and Oregano Oil on Intestinal Injury Caused by Enterotoxigenic Escherichia coli in Weaned Piglets

Table 8

Effects of dietary benzoic acid, bacillus coagulans and oregano oil combined supplementation on serum LPS concentration and DAO activity and jejunal mucosal barrier junction related gene expression of weaned piglets challenged with Escherichia coli.

Items	CON	ETEC	AT	AB	AO	ABO	SEM	P1	P2^†
Serum
LPS EU/L	111.51	186.74	158.08	113.16	104.67	99.69	6.40	<0.001	<0.001
DAO U/mL	31.85	35.02	37.43	37.18	36.01	33.29	0.69	0.269	0.412
Jejunal mucosa
Claudin-1	1.00	0.58	1.12	1.19	1.36	1.51	0.09	0.059	0.045
Occludin	1.00	0.69	1.47	1.18	1.09	1.45	0.08	0.156	0.019
ZO-1	1.00	0.78	1.29	1.09	1.11	1.13	0.05	0.249	0.062
Mucin1	1.00	0.82	1.40	1.45	1.39	1.51	0.10	0.190	0.353
Mucin2	1.00	0.60	1.05	1.17	1.07	1.18	0.06	0.037	0.008

^#Significantly different from CON group (p< 0.05). In the same row, different superscript letters show significant difference among ETEC-challenged groups (p< 0.05).
P1 was used to determine the response to ETEC challenge, CON v. ETEC.
^†P2 was used to determine the response to benzoic acid, bacillus coagulans, and oregano oil combined supplementation among ETEC-challenged piglets.