Research Article
Glucagon-Like Peptide-1 Mediates the Protective Effect of the Dipeptidyl Peptidase IV Inhibitor on Renal Fibrosis via Reducing the Phenotypic Conversion of Renal Microvascular Cells in Monocrotaline-Treated Rats
Figure 3
Effects of sitagliptin, exendin-3, and liraglutide on MCT-induced renal cells apoptosis. (a) Representative renal histological staining with HE, immunohistochemical staining for caspase 3 and proliferating cell nuclear antigen (PCNA), and TUNEL staining. (b) Graphic analysis of immunohistochemistry for caspase 3. (c) Graphic analysis of TUNEL (×200). (d) Graphic analysis of immunohistochemistry for PCNA (×200). (e, f, g, h) Representative western blots and immunoblot analysis of cleaved-caspase 3 (c-caspase 3), glucose-regulated protein 78 (GRP78), Bcl2, and Bax. Data are expressed as the means ± SD; –8 rats in each group; versus control (Con); versus MCT; versus MCT + 40 mg/kg SG.
(a) |
(b) |
(c) |
(d) |
(e) |
(f) |
(g) |
(h) |