Subcritical Water Extraction of Chlorella pyrenoidosa: Optimization through Response Surface Methodology

Table 6

Major compounds in biocrude oil from C. pyrenoidosa at different reaction temperatures.

Order	Retention time (min)	Fatty acid methyl esters (FAMEs)	FAME content (%)
			170°C	220°C	270°C	370°C	370°C
1	6.06	Methyl myristoleate (C14:1)			38.41	4.44	1.97
2	8.78	Methyl palmitate (C16:0)			61.59	5.41
3	9.00	Methyl palmitoleate (C16:1)				69.03
4	12.13	Methyl oleate (C18:1)					0.90
5	12.21	Methyl vaccenate (C18:1)				2.54
6	13.68	Methyl linolenate (C18:3)				8.67
7	15.20	Methyl 11-eicoenoate (C20:1)		9.35
8	15.78	Methyl 11-14 eicosadienoate (C20:2)					62.71
9	16.42	Methyl arachidonate (C20:4)					2.91
10	16.68	Methyl 11-14-17 eicosapentaenoate (C20:3)					7.14
11	17.42	Methyl eicosapentaenoate (C20:5)				4.11	3.91
12	18.11	Methyl erucate (C22:1)					0.90
13	18.81	Methyl lignocerate (C24:0)	100	90.65			0.76
14	20.66	Methyl docosahexaenoate (C22:6)				1.61	3.75
15	20.93	Methyl nervonate (C24:1)				4.19	15.05
		Saturated fatty acids (%)	100	90.65	61.59	5.41	0.76
		Monounsaturated fatty acids (%)		9.35	38.41	80.20	18.82
		Polyunsaturated fatty acids (%)				14.39	80.42