Analysis of In Vitro Cyto- and Genotoxicity of Barbatimão Extract on Human Keratinocytes and Fibroblasts

Figure 1

General experiment design. (A) Initially was obtained a hydroalcoholic extract using barbatimão bark samples. (B) Main bioactive compounds were quantified by HPLC-DA. (C) An exploratory noncelular GEMO assay was conducted to determine if barbatimão at 10 different concentrations could present some genotoxic or genoprotective capacity of the extract. (D) The in vitro protocols were performed in two commercial human cell lines of keratinocytes (HaCaT) and dermal fibroblasts (HFF-1). Additional protocols were conducted to evaluate barbatimão effects on DNA oxidation by quantification of DNA-8-OhdG levels and by intrinsic or extrinsic apoptosis induction by quantification and comparison with negative control group of gene BAX/Bcl-2 ratio and CASP 3 and 8 protein levels. Details of experimental design and assays used in this study are presented in Methods section.