Analysis of In Vitro Cyto- and Genotoxicity of Barbatimão Extract on Human Keratinocytes and Fibroblasts

Figure 3

Barbatimão preliminary assays: (a) genoprotective capacity determined by GEMO noncellular assay that uses DNA PicoGreen® fluorescent dye (Cadoná et al., 2014). This dye presents specific affinity to binding with double-strand (ds) DNA levels. In GEMO assay a solution containing isolated calf dsDNA and H₂O₂ (1M) is produced. The H₂O₂ trigger extensive DNA fragmentation that causes decreasing in the fluorescence and here is considered the control group represented by 0 value. Cell culture supplementation with barbatimão hydroalcoholic extract at different concentrations showed significant increase in the fluorescence indicating genoprotective effect of this extract; barbatimão’s viability effect on (b) keratinocytes and (c) fibroblast 24 h cell cultures measured by MTT-assay. Data were analyzed by ANOVA one-way analysis of variance followed by Tukey post hoc test and all statistical tests with p ≤ 0.05 were considered significant. Statistical differences among treatments were identified by different alphabetical letters, whereas same letters indicated no differences between each treatment compared to the others.

(a)

(b)

(c)