In Vitro Neural Differentiation of Bone Marrow Mesenchymal Stem Cells Carrying the FTH1 Reporter Gene and Detection with MRI

Figure 5

Expression of neuron-specific markers before and after differentiation. (a) Immunofluorescence results for nestin, NSE, and MAP-2 in MSCs and MSCs-TFH1 before induction were negative. After induction, 3 markers in Neurons and Neurons-FTH1 exhibited strong positive expression. (b)-(c) qRT-PCR demonstrated that nestin, NSE, and MAP-2 were not significantly expressed in MSCs and MSCs-FTH1 before induction. After differentiation induction, nestin, NSE, and MAP-2 were significantly expressed in Neurons and Neurons-FTH1, and no significant differences were noted between these two groups (P>0.05).

(a)

(b)

(c)