F806 Suppresses the Invasion and Metastasis of Esophageal Squamous Cell Carcinoma via Downregulating F-Actin Assembly-Related Rho Family Proteins

Figure 3

F806 induces rearrangement of the F-actin cytoskeleton of ECCC cells. (a) The fluorescence value of F-actin (grey peak) and G-actin (white peak) was measured by flow cytometry in F806-treated or control EC109 cells. Data are mean ± SD and P<0.01, compared to the control group; n = 6. (b) EC109 and KYSE510 cells stained with phalloidin were analyzed using an Olympus FV1000 confocal microscope. Scale bar = 10 μm. (c) Exogenous RFP-actin-forming filopodia in KYSE510 cells were visualized and recorded by a confocal microscope with a 40×, 1.43 NA lens, Plan-Apochromat oil objective. (d) The G-actin/F-actin ratio was determined by a G/F-actin in vivo assay kit. C: control; F: F806; T: total protein; S: supernatant; P: pellet.

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