Hydrogen Sulfide Protects against Chemical Hypoxia-Induced Injury via Attenuation of ROS-Mediated Ca²⁺ Overload and Mitochondrial Dysfunction in Human Bronchial Epithelial Cells

Figure 2

Hydrogen sulfide decreased the level of ROS induced by hypoxia in 16HBE14o- cells. Generation of ROS was determined by laser scanning confocal microscope analysis after staining the cells with DCFH-DA. (a, b) 16HBE14o- cells were pretreated with CoCl₂ in varying doses (0, 100, 200, 400, 600, or 1000 μmol/L). (c, d) 16HBE14o- cells were pretreated with CoCl₂ (400 μmol/L) and NaHS (300 μmol/L) separately or together. Results are mean values ± SD of independent experiments performed in triplicate (NS, p < 0.01, p < 0.001 versus control group, ###p < 0.001 versus hypoxia group, n=3).

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