BioMed Research International / 2018 / Article / Fig 4

Research Article

Hydrogen Sulfide Protects against Chemical Hypoxia-Induced Injury via Attenuation of ROS-Mediated Ca2+ Overload and Mitochondrial Dysfunction in Human Bronchial Epithelial Cells

Figure 4

Effects of hydrogen sulfide on MMP induced by hypoxia in 16HBE14o- cells. MMP in 16HBE14o- cells was detected by incubating the cells with Rh123 and analyzed with the fluorescent microscopy software. (a, b) Cells were pretreated with CoCl2 in different concentration (0, 100, 200, 400, 600, or 1000 μmol/L). (c, d) 16HBE14o- cells were pretreated with NaHS (300 μmol/L), NAC (1mM), and CoCl2 (400 μmol/L) separately or together. Experiments were repeated for three times (NS, p < 0.05, p < 0.01, p < 0.001 versus control group, #p < 0.05 versus hypoxia group, n=3).

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