Hydrogen Sulfide Protects against Chemical Hypoxia-Induced Injury via Attenuation of ROS-Mediated Ca²⁺ Overload and Mitochondrial Dysfunction in Human Bronchial Epithelial Cells

Figure 4

Effects of hydrogen sulfide on MMP induced by hypoxia in 16HBE14o- cells. MMP in 16HBE14o- cells was detected by incubating the cells with Rh123 and analyzed with the fluorescent microscopy software. (a, b) Cells were pretreated with CoCl₂ in different concentration (0, 100, 200, 400, 600, or 1000 μmol/L). (c, d) 16HBE14o- cells were pretreated with NaHS (300 μmol/L), NAC (1mM), and CoCl₂ (400 μmol/L) separately or together. Experiments were repeated for three times (NS, p < 0.05, p < 0.01, p < 0.001 versus control group, #p < 0.05 versus hypoxia group, n=3).

(a)

(b)

(c)

(d)