The Oncogenic Role of ARG1 in Progression and Metastasis of Hepatocellular Carcinoma

Figure 2

ARG1 promotes cell proliferation of Huh7 cells. ((a) and (b)) The expression of ARG1 was significantly upregulated in overexpression cell group (OE-ARG1) and inhibited in shRNA-ARG1-2# interference cell group (sh-2#) both at mRNA (a) and protein (b) level. Then, shRNA-ARG1-2# was used in the further experiment. β-Actin mRNA was performed as a control in RT-PCR assay, β-actin was used as a loading control in Western blot assays, and the relative expression level of ARG1 protein was normalized to that in CON group. (c) Determination of arginase activity in ARG1 overexpression cells and knockdown cells. (d) ELISA assay suggests that upregulation of ARG1 reduces the protein level of ARG2 in Huh7 cells, and downregulation of ARG1 also reduces its expression. (e) CCK8 assay showed that overexpression of ARG1 increased cell viability of Huh7 cells, and ARG1 knockdown inhibited cell viability. N=3, <0.05 compared to the OE-NC; <0.05 compared to the sh-NC.

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