Cyclic Stretch Enhances Osteogenic Differentiation of Human Periodontal Ligament Cells via YAP Activation

Figure 3

Cyclic stretch-induced osteogenesis was weakened in the YAP-depleted HPDLCs. (a) Two different siRNA were used to deplete YAP in HPDLCs. The knockdown efficiency was verified by qRT-PCR. , p < 0.001. (b) Depletion of YAP was verified using immunoblot. (c) Quantitative analysis of the blots (in (b)), the data was normalized to the level of GAPDH. , p < 0.001. (d) YAP knockdown decreased the alkaline phosphatase activity of HPDLCs treated with cyclic stretch. (e) The ALP activity of HPDLCs was measured using an ALP reagent kit. The activities were normalized to the protein concentration. , P < 0.05; , P < 0.01. (f) mRNA expression of osteogenesis related genes in YAP-depleted HPDLCs. The relative expression levels of osteopontin (OPN), RUNX2, collagen type 1 (COL1), alkaline phosphatase (ALP), osterix (OSX), and osteocalcin (OCN) were normalized to GAPDH. , p < 0.05; , p < 0.01. All data was based on three independent experiments.

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