Elucidation on Predominant Pathways Involved in the Differentiation and Mineralization of Odontoblast-Like Cells by Selective Blockade of Mitogen-Activated Protein Kinases

Figure 3

Gene expression profile of osteogenesis related markers in culture containing SB202190, SP600125, or PD98059. MDPC-23 cells were cultured as described in Materials and Methods. Total RNA was isolated on day seven and analyzed by reverse-transcription PCR with the indicated primers illustrated in Table 1. Control means cells cultured in the presence of β-GP and AA, without Dex or any inhibitors. Dex group means cells maintained in β-GP, AA, and Dex, without any inhibitors. The concentration for the three inhibitors was unified to be 20 μM (a–e indicate significant differences between different characters in each panel, ).