Elucidation on Predominant Pathways Involved in the Differentiation and Mineralization of Odontoblast-Like Cells by Selective Blockade of Mitogen-Activated Protein Kinases

Table 1

Primers information.

Gene name	Forward	Backward	Fragment size; (bp)	Annealing temperature; (°C)
BSP (NM_012587.2)	CTGCTTTAATCTTGCTCTG	CCATCTCCATTTTCTTCC	211	55
ALP (NM_013059.1)	GGAAGGAGGCAGGATTGACCAC	GGGCCTGGTAGTTGTTGTGAGC	338	55
OPN (NM_012881.2)	TTTCCCTGTTTCTGATGAACAGTAT	CTCTGCTTATACTCCTTGGACTGCT	228	55
Npnt (XM_008761543.1)	CTCAAAGCTGTGTGCCAACC	TTGTGGCTTGATGATCCGGG	178	59.9
Runx-2 (NM_001278484.2)	CCACAGAGCTATTAAAGTGACAGTG	AACAAACTAGGTTTAGAGTCATCAAGC	87	55
DMP-1 (NM_203493.3)	CGTTCCTCTGGGGGCTGTCC	CCGGGATCATCGCTCTGCATC	577	62
BMP-4 (NM_012827.2)	CAGGGCCAACATGTCAGGAT	TGGCGACGGCAGTTCTTATT	188	59.9
COL1A1 (NM_053304.1)	AGAATATGTATCACCAGACG	CAGCTGATTTCTCATCATAG	224	43
OCN (NM_013414.1)	AGCTCAACCCCAATTGTGAC	AGCTGTGCCGTCCATACTTT	190	55
ITGA1 (NM_030994.2)	TCAACGTTAGCCTCACCGTC	CAGGGATCGTCTCATTGGCA	396	59.9
ITGA3 (XM_003750907.2)	GAAAGGCTGACCGACGACTA	TGCGTGGTACTTGGGCATAA	108	66
ITGA5 (NM_001108118.1)	GAAGGGACGGAGTCAGTGTG	TGAATGGTGCTGCACTGGAT	127	66
ITGAV (XM_008762000.1)	ATAAAGCGCGGATGGCAAAG	CTCACCCGAAGATAGGCGAC	213	64.9
ITGB1 (NM_017022.2)	ACAAGAGTGCCGTGACAACT	AGCTTGATTCCAAGGGTCCG	325	59.9
ITGB5 (NM_147139.2)	CACGGTCCATCATCTCTCGG	CATGGAGAGGGAGAGGTCCA	281	62.8
β-actin (NM_031144.3)	AACCCTAAGGCCAACAGTGAAAAG	TCATGAGGTAGTCTGTGAGGT	241	53