MiR-29a Inhibits Glioma Tumorigenesis through a Negative Feedback Loop of TRAF4/Akt Signaling

Figure 5

TRAF4 regulates the expression of miR-29a. (a) U251 cells were transfected with TRAF4 overexpressing plasmid. 24h later, cells were harvested for qRT-PCR to detect the expression level of TRAF4 and miR-29a. (b) Protein expression analysis of TRAF4 and p-Akt was performed by western blot in U251 cells treated as (a). (c) A172 or U251 cells were treated with 20μM LY294002 or 5μg/ml SC79 for 16h. Cells were harvested for western blot to detect the protein level of p-Akt. (d) mRNA expression analysis of miR-29a was performed by qRT-PCR in A172 or U251 cells treated as (c). : P<0.05; : P<0.001 versus control group.

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