Second Blood Meal by Female Lutzomyia longipalpis: Enhancement by Oviposition and Its Effects on Digestion, Longevity, and Leishmania Infection

Table 1

Comparison of conditions and protocols used to study the effect of Leishmania infections on Lutzomyia longipalpis midgut trypsin.

Parameter	Santos et al. 2014	Telleria et al. 2010	Sant’anna et al. 2009	This work
Parasite species	L. infantum	L. (infantum) chagasi	L. mexicana	L. mexicana
Parasite form used in infections	Amastigote	Promastigote	Amastigote	Amastigote
Parasite concentration in infection	Natural infection	10^7 parasites/mL	10^6 parasites/mL	10^6 parasites/mL
Blood source	Dog	Hamster	Rabbit	Sheep
Trypsin substrate	L-BApNA (0.5 mM)	DL-BApNA (2 mM)	BApNA (0.88 mM)	L-BApNA (1 mM)
Assay buffer (pH, final concentration)	Tris/HCl; (pH 7.5, 50 mM)	Tris/HCl; (pH 8.0, 10 mM)	Tris; (pH 8.5, 88 mM)	Tris/HCl; (pH 8.0, 155 mM)
Assay temperature (°C)	30	25	Ambient temperature (~22)	30
Incubation time (minutes)	30	60	5	40
Activity presented in	/midgut	mU/midgut	U/mg protein	mU/gut

One unit of enzymatic activity (U) is the amount of enzyme which releases 1 µmol product/min.