Fermentation Conditions and Media Optimization for Isocitric Acid Production from Ethanol by Yarrowia lipolytica
Table 1
The effect of cultivation parameters on ICA production by Y. lipolytica.
Parameters
Biomass (g/L)
ICA (g/L)
CA (g/L)
ICA/CA ratio
(g/g)
pH
3
8.9 ± 1.4
13.75 ± 2.14
13.89 ± 0.74
1 : 1
0.13
4
10.2 ± 1.6
20.81 ± 3.24
23.11 ± 0.65
1 : 1.1
0.21
4.5
11.2 ± 1.7
37.12 ± 2.67
40.15 ± 1.93
1 : 1.1
0.35
5
10.8 ± 1.6
40.64 ± 1.92
37.90 ± 1.54
1.1 : 1
0.38
5.5
10.8 ± 0.3
50.55 ± 0.64
38.14 ± 4.05
1.3 : 1
0.47
6
11.0 ± 0.3
59.00 ± 3.52
30.60 ± 6.77
1.9 : 1
0.55
6.5
10.1 ± 0.2
48.50 ± 3.54
28.20 ± 0
1.7 : 1
0.48
7
8.1 ± 1.3
12.33 ± 1.98
12.10 ± 1.89
1 : 1
0.17
Oxygen concentration (% from saturation)
5–10
7.6 ± 1.2
4.3 ± 0.6
4.1 ± 0.42
1 : 1
0.10
20–25
10.3 ± 1.6
35 ± 5.44
21 ± 3.27
1.7 : 1
0.33
30–35
10.7 ± 1.7
53.2 ± 8.27
28 ± 1.53
1.9 : 1
0.50
40–45
11.2 ± 1.8
53.68 ± 8.36
27 ± 1.37
2 : 1
0.52
50–55
11.0 ± 0.3
59.0 ± 3.52
29.0 ± 0.27
2 : 1
0.55
60–65
10.4 ± 1.6
46.5 ± 7.23
24.5 ± 0.43
1.9 : 1
0.53
75–80
9.0 ± 1.4
35.3 ± 5.49
19.6 ± 1.63
1.8 : 1
0.45
The experiments were performed at 29°C and dissolved oxygen concentration (pO2) 50% (of saturation); the experiments were performed at 29°C and pH 6; values are mean ± standard deviation ().
