BioMed Research International / 2018 / Article / Fig 1

Research Article

-Hederin Arrests Cell Cycle at G2/M Checkpoint and Promotes Mitochondrial Apoptosis by Blocking Nuclear Factor-B Signaling in Colon Cancer Cells

Figure 1

α-Hederin arrests cell cycle at G2/M checkpoint in IL-6-stimulated SW620 cells. SW620 cells or HCT116 cells were treated with vehicle, IL-6, and/or α-hederin at indicated concentrations for 24 h. (a) CCK-8 assay for evaluating cell viability. Cell viability was expressed as percentage of control. Significance: P<0.05 versus control. (b) Cell cycle analysis by flow cytometry. Percentages of cell cycle distribution at the G0/G1 phase, S phase, and G2/M phase were quantified, respectively. Significance: P<0.05 versus control, #P<0.05 versus IL-6. (c) Real-time PCR analyses of mRNA expression of cyclin B1 and CDK1. Significance: P<0.01 versus control, #P<0.05 versus IL-6, ##P<0.01 versus IL-6. (d) Western blot analyses of protein expression of cyclin B1 and CDK1 with quantification. Significance: P<0.01 versus control, #P<0.05 versus IL-6, ##P<0.01 versus IL-6.
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