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BioMed Research International
Volume 2018, Article ID 2571269, 9 pages
Research Article

Hydrogen Water Drinking Exerts Antifatigue Effects in Chronic Forced Swimming Mice via Antioxidative and Anti-Inflammatory Activities

1Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University, Wonju 26426, Republic of Korea
2Department of Global Medical Science, Wonju College of Medicine, Yonsei University, Wonju 26426, Republic of Korea
3Anydoctor Health Care Co. Ltd. 234, Beotkkot-ro, Geumcheon-gu, Seoul 08513, Republic of Korea
4Mymirae Dermatology Clinic and Hydrogen Skin Research Institute, 7, Gukjegeumyung-ro 2-gil, Yeongdeungpo-gu, Seoul, Republic of Korea
5Department of Microbiology, Wonju College of Medicine, Yonsei University, Wonju 26426, Republic of Korea
6Department of Internal Medicine, Wonju College of Medicine, Yonsei University, Wonju 26426, Republic of Korea
7Institute for Poverty Alleviation and International Development, Yonsei University, 1 Yonseidae-gil, Wonju, Gangwon-do 26493, Republic of Korea

Correspondence should be addressed to Kwang Yong Shim; and Kyu-Jae Lee; moc.liamg@9oibdem

Received 26 September 2017; Revised 19 January 2018; Accepted 13 March 2018; Published 18 April 2018

Academic Editor: Kazim Husain

Copyright © 2018 Jesmin Ara et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Purpose. This study was performed to evaluate antifatigue effect of hydrogen water (HW) drinking in chronic forced exercise mice model. Materials and Methods. Twelve-week-old C57BL6 female mice were divided into nonstressed normal control (NC) group and stressed group: (purified water/PW-treated group and HW-treated group). Stressed groups were supplied with PW and HW, respectively, ad libitum and forced to swim for the stress induction every day for 4 consecutive weeks. Gross antifatigue effects of HW were assessed by swimming endurance capacity (once weekly for 4 wk), metabolic activities, and immune-redox activities. Metabolic activities such as blood glucose, lactate, glycogen, blood urea nitrogen (BUN), and lactate dehydrogenase (LDH) as well as immune-redox activities such as reactive oxygen species (ROS), nitric oxide (NO), glutathione peroxidase (GPx), catalase, and the related cytokines were evaluated to elucidate underlying mechanism. Blood glucose and lactate were measured at 0 wk (before swimming) and 4 wk (after swimming). Results. HW group showed a higher swimming endurance capacity () than NC and PW groups. Positive metabolic effects in HW group were revealed by the significant reduction of blood glucose, lactate, and BUN in serum after 4 wk (, resp.), as well as the significant increase of liver glycogen () and serum LDH () than PW group. In parallel, redox balance was represented by lower NO in serum () and increased level of GPx in both serum and liver () than PW group. In line, the decreased levels of serum TNF-α (), IL-6, IL-17, and liver IL-1β () in HW group revealed positive cytokine profile compared to PW and NC group. Conclusion. This study shows antifatigue effects of HW drinking in chronic forced swimming mice via metabolic coordination and immune-redox balance. In that context, drinking HW could be applied to the alternative and safety fluid remedy for chronic fatigue control.