Acetylation of HMGB1 by JNK1 Signaling Promotes LPS-Induced Peritoneal Mesothelial Cells Apoptosis
Acetylated HMGB1 protein levels in PD patients and LPS-associated peritonitis. (a) PDE samples of PD patients with Gram-negative peritonitis or without were pulled down with antibody against HMGB1 and the immunoprecipitation were probed with anti-acetyl-lysine, then stripped and reprobed for HMGB1. (b) Densitometry of acetyl-HMGB1 in immunoblots (relative to HMGB1). Data are means±SE (n=6). p<0.05 versus control subjects. (c) Visceral peritoneum tissue lysates of control and LPS-treated mice were immunoprecipitated with anti-acetyl-lysine and immunoblotted for HMGB1. Densitometry of acetyl-HMGB1 normalized to HMGB1 in immunoblots. (d) HMrSV5 cell were treated with 5ug/ml of LPS for the indicated time period. Cell culture supernatants were immunoprecipitated with anti-acetylated lysine and probed with anti-HMGB1. The ratio of acetyl-HMGB1 to HMGB1 was quantitatively analyzed. Data in (c) and (d) are means±SE (n=6). p<0.05 versus control group.
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