Identification of Feline Interferon Regulatory Factor 1 as an Efficient Antiviral Factor against the Replication of Feline Calicivirus and Other Feline Viruses

Figure 1

Identification of feline ISGs that inhibit FCV infection. (a) CRFK cells were transfected with a plasmid expressing 10 different ISGs: IRF1, RNase L, SAMHD, ISG15, ISG20, IFIT3, MX-1, GBP6, OASL, and IRF2. At 24 h posttransfection, their expression levels were determined by Western blot using an anti-Flag antibody. (b, c) Effect of ISGs expression on FCV replication. CRFK cells were transfected with 500 ng/well of the ISG plasmid or empty vector or IFN-β expression plasmid (positive control). At 24 h posttransfection, cells were infected with FCV F9 at an MOI of 0.01. At 24 h posttransfection, cellular total RNA was extracted and the relative levels of FCV genome were tested with qRT-PCR (b). The supernatants were collected for the detection of viral titres (c). Error bars represent standard deviations and each sample was run in triplicate.: P<0.05; : P<0.01.

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