BioMed Research International / 2018 / Article / Tab 1 / Research Article
Identification of Feline Interferon Regulatory Factor 1 as an Efficient Antiviral Factor against the Replication of Feline Calicivirus and Other Feline Viruses Table 1 Primers used in this study.
Primer Sequence (5′–3′) Usage Flag-IRF1-F attgcggccgcgATGCCCATCACTCGGATGCGCATGAGA Amplification of IRF1 Flag-IRF1-R atttctagaCTACGGTGCACAAGGAATGGCCTGGAT Myc- IRF1-F attctcgaggtATGCCCATCACTCGGATGCG Myc- IRF1-R attgcggccgcCTACGGTGCACAAGGAATGG GFP- IRF1-F attctcgagATGCCCATCACTCGGATGCGC GFP- IRF1-R attgaattcgCGGTGCACAAGGAATGGCCT Q-GAPDH-F TGACCACAGTCCATGCCATC qRT-PCR for detection of GAPDH Q-GAPDH-R GCCAGTGAGCTTCCCGTTCA Q-FCV-F ATGATTTGGGGTTGTGATGT qRT-PCR for detection of FCV Q-FCV-R TGGGGCTRTCCATGTTGAT Q-IRF1-F GGAAGTGAAGGACCAGAGC qRT-PCR for detection of IRF1 Q-IRF1-R TCCATCGGAGAAGGTATCA qIFITM1 F CACCACCGTGATCAACATCCA qRT-PCR for detection of IFITM1 qIFITM1 R GACTTCACGGAGTAGGCAAAG qViperin F CATGACCGGGGCGAGTACCTG qRT-PCR for detection of Viperin qViperin R GCAAGGATGTCCAAATATTCACC qISG15 F TCCTGGTGAGGAACCACAAGGG qRT-PCR for detection of ISG15 qISG15 R TTCAGCCAGAACAGGTCGTC
