A Comparative Study of Three Detection Techniques for Leifsonia xyli Subsp. xyli, the Causal Pathogen of Sugarcane Ratoon Stunting Disease

Figure 2

Agarose gel electropherograms of conventional PCR products of DNA extract from Lxx-infected sugarcane juice. Lane M: 100 bp molecular marker; Lane 1: ddH₂O; Lane 2: 20 ng/μL negative DNA extracted from Lxx-free juice; Lanes 3 to 10: 10-fold serial dilutions of DNA (10⁰ to 10⁻⁷, 4.0 ng/μL to 4.0 × 10⁻⁷ ng/μL) extracted from Lxx-infected sugarcane juice. The red arrow points to the limiting detection concentration of Lxx-infected sugarcane juice DNA.