A Comparative Study of Three Detection Techniques for Leifsonia xyli Subsp. xyli, the Causal Pathogen of Sugarcane Ratoon Stunting Disease

Table 2

Ct values of RT-qPCR using serial dilutions of DNA extracted from Lxx-infected sugarcane juice as templates.

Sample	2013.5.		2013.6.2
		Mean Ct	Ct	Mean Ct
Black (ddH2O)	—	—	—	—
Negative juice DNA	—	—	—	—
10^0 positive juice DNA	25.6029; 25.1696; 25.139	25.304	24.6533; 23.8346; 24.3586	24.2822
10^−1 positive juice DNA	29.1754; 28.7072; 28.249	28.711	27.7062; 27.4855; 27.4397	27.5438
10^−2 positive juice DNA	32.437; 32.138; 32.4321	32.336	31.3557; 32.5417; —	31.9487
10^−3 positive juice DNA	—	—	—	—
10^−4 positive juice DNA	—	—	—	—
10^−5 positive juice DNA	—	—	—	—
10^−6 positive juice DNA	—	—	—	—
10^−7 positive juice DNA	—	—	—	—

Results of experiments conducted on 04/25/2013.
Results of experiments conducted on 06/26/2013.
“Ct” means cycle threshold.
“—” means absence detection of Lxx.