Early Enhanced Leucine-Rich α-2-Glycoprotein-1 Expression in Glomerular Endothelial Cells of Type 2 Diabetic Nephropathy Model Mice

Figure 1

Glomerular hypertrophy with endothelial cell proliferation in db/db mice. Representative image of PAS staining in glomeruli of db/m and db/db mice at (a) 16 weeks and (b) 24 weeks of age. The glomerular area was increased in db/db mice at both ages (25 glomeruli per mice were measured and averaged). Values are expressed as the mean ± SEM (n = 4–6 in each group). P < 0.05 versus db/m, unpaired t-test. Original magnification: ×200, bars = 20 μm. Representative image of CD34 immunostaining at (c) 16 weeks and (d) 24 weeks of age. Quantitative analysis of CD34 in 350 glomeruli identified by an LMD method at (e) 16 weeks and (f) 24 weeks of age. Values are expressed as the mean ± SEM (n = 4–6 in each group). P < 0.05 versus db/m, unpaired t-test.

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