Optimization of the Cytotoxic Activity of Three Streptomyces Strains Isolated from Guaviare River Sediments (Colombia, South America)

Table 6

Experimental design central composite design representing the cytotoxic percent by Streptomyces strains influenced by four variables and five levels of each on A: carbon source concentration, B: nitrogen source concentration, and C: pH.

Run	Independent variables			Cytotoxicity (100 ppm)
				Observed value			Predicted Value
	A	B	C	73	386	519	73	386	519
	(w/v)	(w/v)		Against PC3	Against A549	Against MDA	Against PC3	Against A549	Against MDA
1	2.5	2	6	78.05	83.482	73.204	77.319	84.139	71.740
2	2.5	0.4	8	75.075	74.138	72.774	73.985	76.455	69.707
3	0.5	2	8	71.182	82.695	87.936	70.728	84.274	85.239
4	0.5	0.4	6	75.075	75.54	74.374	75.060	75.090	75.003
5	0.1	0.8	7	74.44	81.127	72.715	73.952	80.956	74.620
6	5	0.8	7	76.138	81.127	72.715	76.401	80.439	74.271
7	1.4	0.1	7	70.717	81.705	72.834	70.258	81.766	70.462
8	1.4	4	7	72.547	77.078	75.952	72.753	76.486	76.688
9	1.4	0.8	5	72.547	77.078	75.952	72.044	77.487	75.054
10	1.4	0.8	9	74.824	83.369	72.434	74.720	81.934	74.001
11	1.4	0.8	7	73.085	80.37	73.929	73.382	80.819	74.528
12	1.4	0.8	7	70.717	80.078	72.709	73.382	80.819	74.528
13	1.4	0.8	7	72.767	81.494	74.946	73.382	80.819	74.528
14	1.4	0.8	7	72.767	81.568	72.918	73.382	80.819	74.528
15	1.4	0.8	7	74.198	82.272	74.033	73.382	80.819	74.528