HPV16-E6 Oncoprotein Activates TGF- and Wnt/-Catenin Pathways in the Epithelium-Mesenchymal Transition of Cataracts in a Transgenic Mouse Model

Figure 4

The E6 oncoprotein induces the expression of EMT markers. (a) Representative images for E-cadherin mRNA detection by in situ RT-PCR in 2-month-old NT and K14E6 lenses (see Materials and Methods). (b) E-cadherin and vimentin protein expression normalised with GAPDH by Western blot. (c) Immunofluorescence detection of E-cadherin in 2-month-old NT and K14E6 transgenic lens. Green signal: E-cadherin. Blue signal: TO-PRO (cell nuclei). White arrows show nucleated cells that lack E-cadherin signal near the lens nucleus. Statistical significance: < 0.001. Magnifications: (c) (40x).

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