HPV16-E6 Oncoprotein Activates TGF- and Wnt/-Catenin Pathways in the Epithelium-Mesenchymal Transition of Cataracts in a Transgenic Mouse Model

Table 1

Sequence of primers used for genotyping, RT-PCR, and RT-qPCR procedures.

Gene symbol	Forward	Reverse
E6/E7 (gene)	TTTTATGCACCAAAAGAGAACT	TACCTGCAGGATCAGCCATG
E6/E7 (mRNA)	TTTTATGCACCAAAAGAGAACT	CAGCATATGGATTCCCATCTC
p21/WAF1	TTCAGAGCCCAGGCACCATG	GGGACCCAGGGCTCAGGTAGA
COL1A1	ATGTTCAGCTTTGTGGACCTC	AGTTTGAAGCACAGCACTCG
FN1	TGCCTCGGGAATGGAAAG	ATGGTAGGTCTTCCCATCGCTATA
MMP2	TCAAGGACCGGTTTATTTGGC	GCGTCAATCTTTTCTGGGAGC
MMP9	TCCAGCGTGCCGGAAG	CCACGACCATACAGATACTGGATG
Wnt1	CAGGGTTCATAGCGATCCAT	CAAAGAGGGAGGGAGGTAGG
Wnt4	CTGGAGAAGTGTGGCTGTGA	GGACGTCCACAAAGGACTGT
Wnt5a	CTGGCAGGACTTTCTCAAGG	GTCTCTCGGCTGCCTATTTG
MTA1	AGCCCAACCCAAACCAG	GGCAATGCGTGTCAACT
E-cadherin	CAGTTCCGAGGTCTACACCTT	TGAATCGGGAGTCTTCCGAAAA
GAPDH	GGTGAAGGTCGG TGTGAACG	CTCGCTCCTGGAAGATGGTG

Amplification conditions for PCR, RT-PCR, and RT-qPCR were 95°C, 30 sec; 60°C, 60 sec; and 72°C, 30 sec; 40 cycles.
Amplification conditions for in situ RT-PCR were 90°C, 30 sec; 60°C, 30 sec; and 72°C, 30 sec; 20 cycles.