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BioMed Research International
Volume 2018, Article ID 2863068, 10 pages
Research Article

“Positive Regulation of RNA Metabolic Process” Ontology Group Highly Regulated in Porcine Oocytes Matured In Vitro: A Microarray Approach

1Department of Histology and Embryology, Poznan University of Medical Sciences, Swiecickiego 6 St., 60-781 Poznan, Poland
2Department of Anatomy, Poznan University of Medical Sciences, Swiecickiego 6 St., 60-781 Poznan, Poland
3Department of Biomaterials and Experimental Dentistry, Poznan University of Medical Sciences, Bukowska 70 St., 60-812 Poznan, Poland
4Department of Obstetrics and Gynecology, University Hospital and Masaryk University, Obilni trh 11, 602 00 Brno, Czech Republic
5Veterinary Center, Nicolaus Copernicus University in Torun, Gagarina 11 St., 87-100 Torun, Poland
6Department of Histology and Embryology, Wroclaw University of Medical Sciences, 6a Chalubinskiego St., 50-368 Wroclaw, Poland

Correspondence should be addressed to Bartosz Kempisty; lp.ude.pmu@ytsipmekb

Received 11 May 2017; Revised 10 July 2017; Accepted 1 November 2017; Published 10 January 2018

Academic Editor: Heide Schatten

Copyright © 2018 Piotr Celichowski et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The cumulus-oocyte complexes (COCs) growth and development during folliculogenesis and oogenesis are accompanied by changes involving synthesis and accumulation of large amount of RNA and proteins. In this study, the transcriptomic profile of genes involved in “oocytes RNA synthesis” in relation to in vitro maturation in pigs was investigated for the first time. The RNA was isolated from oocytes before and after in vitro maturation (IVM). Interactions between differentially expressed genes/proteins belonging to “positive regulation of RNA metabolic process” ontology group were investigated by STRING10 software. Using microarray assays, we found expression of 12258 porcine transcripts. Genes with fold change higher than and with corrected value lower than 0.05 were considered as differentially expressed. The ontology group “positive regulation of RNA metabolic process” involved differential expression of AR, INHBA, WWTR1, FOS, MEF2C, VEGFA, IKZF2, IHH, RORA, MAP3K1, NFAT5, SMARCA1, EGR1, EGR2, MITF, SMAD4, APP, and NR5A1 transcripts. Since all of the presented genes were downregulated after IVM, we suggested that they might be significantly involved in regulation of RNA synthesis before reaching oocyte MII stage. Higher expression of “RNA metabolic process” related genes before IVM indicated that they might be recognized as important markers and specific “transcriptomic fingerprint” of RNA template accumulation and storage for further porcine embryos growth and development.