miR-34a Inhibits Cell Proliferation by Targeting SATB2 in Hepatocellular Carcinoma

Figure 3

miR-34a targets SATB2 in HCC cells. (a) Predicted miR-34a target sites in the 3’UTR of STAB2. (b) The relative luciferase activities of STAB2-wt, STAB2-mut were measured using a Dual-Luciferase Reporter Assay Kit following transfection with miR-34a mimic (50 pmol/ ml). (c) RT-qPCR was applied to check the expression of SATB2 mRNA. (d) Western blotting was applied to check the expression of SATB2 protein. (e) The relative intensity of SATB2 protein was shown as a bar graph. < 0.05 and < 0.01 versus control group.

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