Whole Peptidoglycan Extracts from the Lactobacillus paracasei subsp. paracasei M5 Strain Exert Anticancer Activity In Vitro

Figure 7

Expression of pro- and antiapoptotic genes in HT-29 cells following exposure to WPG for 48 h. (a) Semiquantitative RT-PCR analysis of apoptotic signaling genes. Column 1, control cells treated with purified water; Column 2, 80 μg mL⁻¹ of WPG treated cells; Column 3, 160 μg mL⁻¹; Column 4, 320 μg mL⁻¹; Column 5, 1000 μg mL⁻¹. Results were confirmed by several separate experiments and representative images are shown. (b) Quantitative expression of apoptotic signaling genes (Bax, Bcl-xl, Bad, and caspase-3) compared with the housekeeping gene β-actin (expression of β-actin was considered 100%). Values with different letters on the columns are significantly different ().

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