Whole Peptidoglycan Extracts from the Lactobacillus paracasei subsp. paracasei M5 Strain Exert Anticancer Activity In Vitro

Figure 8

Expression of Cyto-C gene in HT-29 cells following exposure to WPG for 24 or 48 h. Semiquantitative RT-PCR analysis of Cyto-C gene. Column 1, control cells treated with purified water; Column 2, 80 μg mL⁻¹ of WPG treated cells; Column 3, 160 μg mL⁻¹; Column 4, 320 μg mL⁻¹; Column 5, 1000 μg mL⁻¹. Results were confirmed by several separate experiments and representative images are shown. Quantitative expression of Cyto-C gene compared with the housekeeping gene β-actin (expression of β-actin was considered 100%). Values with different letters on the columns are significantly different ().