Single sample inclusion assessment for the identification of differentially expressed genes. All possible three (top panel) or two (bottom panel) cancer RNA-seq data combinations were used to identify differentially expressed genes. The x-axis shows the number of identified differentially expressed genes (DEGs) and the y-axis indicates the RNA-seq sample included in all possible combinations. The box plots show the number of DEGs identified in a given comparison setting. Centerlines show the medians and box limits indicate the 25th and 75th percentiles, as determined by R software; the whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles. The top panel has n = 36 sample points and the bottom panel has n = 9 sample points.