Identification of Forensically Important Calliphoridae and Sarcophagidae Species Collected in Korea Using SNaPshot Multiplex System Targeting the Cytochrome c Oxidase Subunit I Gene

Table 4

Expected nucleotide base, dye color, expected and observed peak size, range of obtained peak sizes, and standard deviation for Calliphoridae species-specific SNPs.

Fly species (Calliphoridae)

Callipho 90

Callipho 72

Callipho 168

Callipho 261

Callipho 252

Callipho 243

Color

Nucleotide base

Color

Nucleotide base

Color

Nucleotide base

Color

Nucleotide base

Color

Nucleotide base

Color

Nucleotide base

Lucilia ampullacea (Villeneuve, 1922)

A

C

T

C

G

T

Lucilia caesar (Linnaeus, 1758)

A

C

T

C

A

T

Lucilia illustris (Meigen, 1826)

A

C

T

T

A

T

Chrysomya megacephala (Fabricius, 1794)

A

C

A

T

G

T

Chrysomya pinguis (Walker, 1858)

A

C

A

T

A

T

Triceratopyga calliphoroides (Rohdendorf, 1931)

A

A

T

T

A

C

Calliphora lata (Coquillett, 1898)

A

A

T

T

A

T

Phormia regina (Meigen, 1826)

A

T

A

A

A

T

Calliphora vicina (Robineau-Desvoidy, 1830)

A

G

T

T

A

C

Lucilia sericata (Meigen, 1826)

T

C

T

T

A

T

Aldrichina grahami (Aldrich, 1930)

T

A

C

C

A

C

Expected peak size

25

35

45

55

65

75

Observed peak size average

28.73

38.21

48.59

57.79

67.66

78.61

Observed peak size-range ()

27.96–30.59

37.52–39.37

47.80–49.17

56.93–58.47

66.73–68.32

76.68–79.21

STD ()

0.55

0.49

0.40

0.35

0.36

0.39