A Single Codon Optimization Enhances Recombinant Human TNF-α Vaccine Expression in Escherichia coli

Figure 3

The secondary mRNA structure of vaccines rTNF-PADRE and mrTNF-PADRE. (a) The mRNA structure of rTNF-PADRE gene. (b) The mRNA structure of codon optimized mrTNF-PADRE gene in which a synonymous mutation was introduced. Black box showed the partially magnified structures and red box showed the synonymous mutation (TCT→AGT which encodes number 147 amino acid residue of wild-type human TNF-α).

(a)

(b)