A Single Codon Optimization Enhances Recombinant Human TNF-α Vaccine Expression in Escherichia coli

Table 1

Nucleotide sequence of DNA fragments for (m)rTNF-PADRE construction.

Name	Nucleotide sequence
PTF	5′-AATTGGCTAAATTCGTTGCTGCATGGACTCTGAAAGCTGCTCTCGACTTTGCCGAGTCTGGGCAGGTCTACTTTGGGATCATTGCCCTGTGAG-3′
PTR	5′-TCGACTCACAGGGCAATGATCCCAAAGTAGACCTGCCCACTCTCGGCAAAGTCGAGAGCAGCTTTCAGAGTCCATGCAGCAACGAATTTAGCC-3′
P1	5′-GCCATATGGTCAGATCATCTTCTCG-3′
P2	5′-GCGCAATTGGAAGACCCCTC-3′
P3	5′-GCCATATGGTCAGATCATC TTCTCG-3′
P4	5′-GTCGACTCACAGGGCAATGATCCCAAAGTAGACCTGCCCCTCGGC AAAGTCG-3′