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BioMed Research International
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2018
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Article
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Tab 3
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Research Article
Prevalence, Genetic Heterogeneity, and Antibiotic Resistance Profile of
Listeria
spp. and
Listeria monocytogenes
at Farm Level: A Highlight of ERIC- and BOX-PCR to Reveal Genetic Diversity
Table 3
PCR conditions for BOX-PCR.
PCR steps
Temperature (°C)
Duration (min)
Cycle
Initial denaturation
94
5.00
-
Denaturation
94
1.00
35
Annealing
40
2.00
Elongation
72
2.00
Final Extension
72
10.00
-