S-Allyl Cysteine Alleviates Hydrogen Peroxide Induced Oxidative Injury and Apoptosis through Upregulation of Akt/Nrf-2/HO-1 Signaling Pathway in HepG2 Cells

Figure 3

SAC inhibits H₂O₂ induced oxidative injury in HepG2 cells. (a) ROS levels were measured by DCFH₂-DA staining followed by FACS analysis. Cells were either left untreated or treated with SAC for 2 h followed by treatment with 1 mM H₂O₂ in the presence of SAC for 2 h. Fluorescence intensities were plotted as fold difference compared to control. (b) Cells were treated with 1 mM H₂O₂ for 30 minutes and washed off with PBS and then treated with SAC for different time points. ROS was measured by DCFH₂-DA staining followed by FACS analysis. Fluorescence intensities were plotted as fold difference compared to the respective H₂O₂ treated control. (c) Lipid peroxidation was assessed by measuring MDA levels by TBARS method. Cells were treated with 1 mM H₂O₂ for 6 h with or without treatment with the indicated concentrations of SAC. MDA levels are represented as fold change over control. P<0.01 and P<0.001 compared to the control group. #P<0.05, ##P<0.01, and ###P<0.001 compared to the H₂O₂ treated group.

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