Cytotoxic and Apoptotic Effects of Govaniadine Isolated from Corydalis govaniana Wall. Roots on Human Breast Cancer (MCF-7) Cells

Figure 5

Cell apoptosis observed using fluorescence microscope (200×). Cells were treated with different dose of govaniadine for 24 h and stained with (row (a)) acridine orange-ethidium bromide and (row (b)) Hoechst 33258. (A and A′) untreated cells (0.1% DMSO), (B and B′) cells treated with 2 μM of govaniadine, (C and C′) cells treated with 4 μM of govaniadine, (D and D′) cells treated with 8 μM of govaniadine, and (E and E′) cells treated with 16 μM of govaniadine. Scale bar: 100 μm.

(a) AO/EB staining

(b) Hoechst 33258 staining