Cytotoxic and Apoptotic Effects of Govaniadine Isolated from Corydalis govaniana Wall. Roots on Human Breast Cancer (MCF-7) Cells

Figure 8

Induction of apoptosis in MCF-7 cells by govaniadine determined by Annexin V-PI flow cytometry technique. (a) Control (0.1% DMSO), (b), (c), and (d) treated with govaniadine 4, 8, and 16 μM, respectively. The lower left quadrant represents intact viable cells (Annexin-FITC and PI negative). The upper left quadrant represents early apoptotic cells (Annexin-FITC positive and PI negative). The upper right region represents late apoptotic cells (Annexin-FITC negative and PI positive). The lower left quadrant represents necrotic cells (Annexin-FITC and PI positive). The data are presented as dot plots of Annexin V/FITC against PI of at least three independent tests. Data were analyzed by specific software, FCS Express 5.

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