Site-Directed Spin Labeling EPR for Studying Membrane Proteins

Figure 6

CW dipolar broadening EPR study of KCNE1. (a) CW dipolar broadening EPR spectra of KCNE1 bearing BSLs at sites 53/57 and 63/67 in POPC/POPG liposomes (left panel) and the corresponding distance distribution (right panel) obtained from data analysis by using the Short Distances LabVIEW program. (b) Cartoon representation of KCNE1 bearing two BSLs at sites 53/57 and 63/67 (left panel) and the corresponding distance distribution obtained from 20 ns molecular dynamics trajectory data analysis (right panel) (adapted from [53] with permission).

(a)

(b)