Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2018, Article ID 3274641, 7 pages
https://doi.org/10.1155/2018/3274641
Research Article

miR-142-5p in Bone Marrow-Derived Mesenchymal Stem Cells Promotes Osteoporosis Involving Targeting Adhesion Molecule VCAM-1 and Inhibiting Cell Migration

1Department of Orthopedic Surgery, The People’s Hospital of Yuxi City, The 6th Affiliated Hospital of Kunming Medical University, Yuxi, Yunnan 653100, China
2Huai’an Second People’s Hospital, The Affiliated Huai’an Hospital of Xuzhou Medical University, Huai’an, Jiangsu 223200, China
3Health Screening Center, The People’s Hospital of Yuxi City, The 6th Affiliated Hospital of Kunming Medical University, 21 Nieer Road, Yuxi, Yunnan 653100, China
4Department of Orthopedics, The First People’s Hospital of Kunming, Kunming, Yunnan 650011, China
5Department of Orthopedics, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150006, China
6Department of Orthopedics, Affiliated Hospital of Hebei University of Engineering, Handan, Hebei 056002, China
7Sansom Institute for Health Research, School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5001, Australia

Correspondence should be addressed to Xiguang Zhang; moc.361@gxzkwg and Cory J. Xian; ua.ude.asinu@naix.yroc

Received 2 November 2017; Revised 27 January 2018; Accepted 28 February 2018; Published 29 March 2018

Academic Editor: Weijie Fu

Copyright © 2018 Zhaowei Teng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Osteoporosis is a systemic bone metabolic disease that is highly prevalent in the elderly population, particularly in postmenopausal women, which results in enhanced bone fragility and an increased susceptibility to fractures. However, the underlying molecular pathogenesis mechanisms still remain to be further elucidated. In this study, in a rat ovariectomy- (OVX-) induced postmenopausal osteoporosis model, aberrant expression of a microRNA miR-142-5p and vascular cell adhesion molecule 1 (VCAM-1) was found by RNA sequencing analysis and qRT-PCR. Using a dual-luciferase reporter assay, we found that miR-142-5p can bind to and decrease expression of VCAM-1 mRNA. Such reduction was prohibited when the miR-142-5p binding site in VCAM-1 3′UTR was deleted, and Western blotting analyses validated the fact that miR-142-5p inhibited the expression of VCAM-1 protein. Bone marrow-derived mesenchymal stem cells (BMMSCs) transfected with miR-142-5p showed a significantly decreased migration ability in a Transwell migration assay. Collectively, these data indicated the important role of miR-142-5p in osteoporosis development involving targeting VCAM-1 and inhibiting BMMSC migration.