miR-142-5p in Bone Marrow-Derived Mesenchymal Stem Cells Promotes Osteoporosis Involving Targeting Adhesion Molecule VCAM-1 and Inhibiting Cell Migration

Figure 3

miR-142-5p can inhibit the expression of VCAM-1. (a) Results of VCAM-1 in BMMSCs transcriptome sequencing. (b) A schematic diagram of miR-142-5p paring with the 3′UTR area of VCAM-1, as well as the schematic diagram of the luciferase expression vector constructs, respectively, with wild-type (WT) and mutant (Mut) VCAM-1 sequences. (c) Test results of the dual-luciferase activity assay; for each group. (d) Transfection with the miR-142-5p mimic, but not the negative control (NC) mimic, inhibits protein expression of VCAM-1 as revealed by Western blotting analyses. .

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