Saccharomyces cerevisiae-Derived Mannan Does Not Alter Immune Responses to Aspergillus Allergens

Figure 1

Airway inflammation after allergen exposure was reduced by MN treatment. Cells in the bronchoalveolar lavage (BAL) were analyzed by flow cytometry. Single cells expressing Siglec-F and CD193 were considered eosinophils (Eos), while Ly6G expressing cells were considered neutrophils (Neuts). Siglec-F expressing cells with high forward scatter were considered to be macrophages (Macs). CD3ε⁺CD4⁺ lymphocytes (Lymphs) that expressed CD193 were considered to be cells (a). The total number of cells in the BAL was enumerated and the subpopulations were determined as a percentage from the total (b). MN treatment alone led to an inflammatory response in the airways but MN treatment during allergic sensitization and challenge resulted in a reduction of allergic inflammation (b). The influx of cells into the airways was induced in MN-treated allergic mice (b). Data are represented as the mean and standard deviation in –10 mice per group. Data in each group were analyzed by one-way ANOVA with Dunn’s multiple comparisons test and denotes and denotes .

(a)

(b)